Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course.

ABSTRACT: Inquiry-driven lab exercises require students to think carefully about a question, carry out an investigation of that question, and critically analyze the results of their investigation. Here, we describe the implementation and assessment of an inquiry-based laboratory exercise in which students obtain and analyze novel data that contribute to our understanding of macromolecular trafficking between the nucleus and cytoplasm in eukaryotic cells. Although many of the proteins involved in nucleocytoplasmic transport are known, the physical interactions between some of these polypeptides remain uncharacterized. In this cell and molecular biology lab exercise, students investigate novel protein-protein interactions between factors involved in nuclear RNA export. Using recombinant protein expres

SUBMITTER: Belanger KD 

PROVIDER: S-EPMC2736025 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature