Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis.

ABSTRACT: Increasing evidence indicates that an important consequence of protein posttranslational modification (PTM) is the creation of a high affinity binding site for the selective interaction with a PTM-specific binding protein (BP). This PTM-mediated interaction is typically required for downstream signaling propagation and corresponding biological responses. Because the vast majority of mammalian proteins contain PTMs, there is an immediate need to discover and characterize previously undescribed PTMBPs. To this end, we developed and validated an innovative in vivo approach called mammalian tethered catalysis (MTeC). By using methylated histones and methyl-specific histone binding proteins as the proof-of-principle, we determined that the new MTeC approach can compliment existing in vitro bind

SUBMITTER: Spektor TM 

PROVIDER: S-EPMC2736425 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature