Biochemical basis for the essential genetic requirements of RecA and the beta-clamp in Pol V activation.

ABSTRACT: In Escherichia coli, it is genetically well established that the beta-clamp and RecA are essential cofactors that endow DNA polymerase (Pol) V with lesion bypass activity. However, the biochemical basis for these requirements is still largely unknown. Because the process of translesion synthesis (TLS) requires that the specialized DNA polymerase synthesize in a single binding event a TLS patch that is long enough to resist external proofreading, it is critical to monitor Pol V burst synthesis. Here, we dissect the distinct roles that RecA and the beta-clamp perform during the Pol V activation process using physiologically relevant long single-stranded template DNA, similar to those used in genetic assays. Our data show that the beta-clamp endows the complex between Pol V and the template D

SUBMITTER: Fujii S 

PROVIDER: S-EPMC2736454 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature