The role of apoptotic cell death in the radiosensitising effect of gemcitabine.

ABSTRACT:

Background

The aim of this study was to evaluate the radiosensitising effect of gemcitabine, in terms of cell-cycle progression, induction of apoptosis, and to investigate the molecular events regulating apoptosis.

Methods

Tumour cells were treated with gemcitabine, radiation, or the combination. 0-72 h after treatment, cells were collected for cell-cycle analysis and apoptosis determination. Caspase 8 and 9, Bid and tBid expression were determined by western blot. The mitochondrial membrane potential was determined using flow cytometry. An RT(2) Profiler PCR Array for human apoptotic genes was performed after the combination or TRAIL treatment.

Results

Gemcitabine and radiation resulted in an early S-phase block immediately after treatment, after which the cells move

SUBMITTER: Pauwels B 

PROVIDER: S-EPMC2736812 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature