Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer.

ABSTRACT:

Background

Retinoic acid-regulated nuclear matrix-associated protein (RAMP) is a WD40 repeat-containing protein that is involved in various biological functions, but little is known about its role in human cancer. This study aims to delineate the oncogenic role of RAMP in gastric carcinogenesis.

Methods

RAMP expression was examined by real-time quantitative RT-PCR, immunohistochemistry and western blotting. Inhibition of RAMP expression was performed by siRNA-mediated knockdown. The functional effects of RAMP on cell kinetics were measured by cell viability assay, colony formation assay and flow cytometry. Cell lines stably expressing RAMP were established to investigate the oncogenic effects of RAMP in vitro.

Results

Ramp was readily expressed in all seven gastric ca

SUBMITTER: Li J 

PROVIDER: S-EPMC2736823 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature