A possibilistic framework for constraint-based metabolic flux analysis.

ABSTRACT:

Background

Constraint-based models allow the calculation of the metabolic flux states that can be exhibited by cells, standing out as a powerful analytical tool, but they do not determine which of these are likely to be existing under given circumstances. Typical methods to perform these predictions are (a) flux balance analysis, which is based on the assumption that cell behaviour is optimal, and (b) metabolic flux analysis, which combines the model with experimental measurements.

Results

Herein we discuss a possibilistic framework to perform metabolic flux estimations using a constraint-based model and a set of measurements. The methodology is able to handle inconsistencies, by considering sensors errors and model imprecision, to provide rich and reliable flux estimations.

SUBMITTER: Llaneras F 

PROVIDER: S-EPMC2736929 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature