Genomic analysis of a sexually-selected character: EST sequencing and microarray analysis of eye-antennal imaginal discs in the stalk-eyed fly Teleopsis dalmanni (Diopsidae).

ABSTRACT:

Background

Many species of stalk-eyed flies (Diopsidae) possess highly-exaggerated, sexually dimorphic eye-stalks that play an important role in the mating system of these flies. Eye-stalks are increasingly being used as a model system for studying sexual selection, but little is known about the genetic mechanisms producing variation in these ornamental traits. Therefore, we constructed an EST database of genes expressed in the developing eye-antennal imaginal disc of the highly dimorphic species Teleopsis dalmanni. We used this set of genes to construct microarray slides and compare patterns of gene expression between lines of flies with divergent eyespan.

Results

We generated 33,229 high-quality ESTs from three non-normalized libraries made from the developing eye-stalk ti

SUBMITTER: Baker RH 

PROVIDER: S-EPMC2737001 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature