Nucleotide identity and variability among different Pakistani hepatitis C virus isolates.

ABSTRACT:

Background

The variability within the hepatitis C virus (HCV) genome has formed the basis for several genotyping methods and used widely for HCV genotyping worldwide.

Aim

The aim of the present study was to determine percent nucleotide identity and variability in HCV isolates prevalent in different geographical regions of Pakistan.

Methods

Sequencing analysis of the 5'noncoding region (5'-NCR) of 100 HCV RNA-positive patients representing all the four provinces of Pakistan were carried out using ABI PRISM 3100 Genetic Analyzer.

Results

The results showed that type 3 is the predominant genotypes circulating in Pakistan, with an overall prevalence of 50%. Types 1 and 4 viruses were 9% and 6% respectively. The overall nucleotide similarity among different Pakistan

SUBMITTER: Idrees M 

PROVIDER: S-EPMC2737312 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature