A novel tryptophanyl-tRNA synthetase gene confers high-level resistance to indolmycin.

ABSTRACT: Indolmycin, a potential antibacterial drug, competitively inhibits bacterial tryptophanyl-tRNA synthetases. An effort to identify indolmycin resistance genes led to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase. Overexpression of this gene in an indolmycin-sensitive strain increased the indolmycin MIC 60-fold. Its transcription and distribution in various bacterial genera were assessed. The level of resistance conferred by this gene was compared to that of a known indolmycin resistance gene and to those of genes with resistance-conferring point mutations.

SUBMITTER: Vecchione JJ 

PROVIDER: S-EPMC2737876 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature