Role of the type III secretion system in a hypervirulent lineage of Bordetella bronchiseptica.

ABSTRACT: Despite the fact that closely related bacteria can cause different levels of disease, the genetic changes that cause some isolates to be more pathogenic than others are generally not well understood. We use a combination of approaches to determine which factors contribute to the increased virulence of a Bordetella bronchiseptica lineage. A strain isolated from a host with B. bronchiseptica-induced disease, strain 1289, was 60-fold more virulent in mice than one isolated from an asymptomatically infected host, strain RB50. Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secretion system (TTSS) genes were more highly expressed by strain 1289 than strain RB50. Compared to strain RB50, strain 1289 exhibited greater TTSS-mediated cytotoxicity of a mamm

SUBMITTER: Buboltz AM 

PROVIDER: S-EPMC2738013 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature