Gene expression profiling of multiple leiomyomata uteri and matched normal tissue from a single patient.

ABSTRACT:

Objective

To identify differentially expressed genes between fibroid and adjacent normal myometrium in an identical hormonal and genetic background.

Design

Array analysis of three leiomyomata and matched adjacent normal myometrium in a single patient.

Setting

University of Colorado Hospital.

Patient(s)

A single female undergoing medically indicated hysterectomy for symptomatic fibroids.

Interventions(s)

mRNA isolation and microarray analysis, reverse-transcriptase polymerase chain reaction, Western blotting, and immunohistochemistry.

Main outcome measure(s)

Changes in mRNA and protein levels in leiomyomata and matched normal myometrium.

Result(s)

Expression of 197 genes was increased and 619 decreased significantly by at least twofold, in l

SUBMITTER: Dimitrova IK 

PROVIDER: S-EPMC2738624 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature