Evaluation and validation of a real-time PCR assay for detection and quantitation of human adenovirus 14 from clinical samples.

ABSTRACT: In 2007, the Centers for Disease Control and Prevention (CDC) reported that Human adenovirus type 14 (HAdV-14) infected 106 military personnel and was responsible for the death of one U.S. soldier at Lackland Air Force Base in Texas. Identification of the responsible adenovirus, which had not previously been seen in North America and for which rapid diagnostic tools were unavailable, required retrospective analysis at reference laboratories. Initial quarantine measures were also reliant on relatively slow traditional PCR analysis at other locations. To address this problem, we developed a real-time PCR assay that detects a 225 base pair sequence in the HAdV-14a hexon gene. Fifty-one oropharyngeal swab specimens from the Naval Health Research Center, San Diego, CA and Advanced Diagnostic La

SUBMITTER: Metzgar D 

PROVIDER: S-EPMC2738968 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature