In vivo microcartography and subcellular imaging of tumor angiogenesis: a novel platform for translational angiogenesis research.

ABSTRACT:

Purpose

To eliminate the variable of tumor heterogeneity from a novel in vivo model of tumor angiogenesis.

Experimental design

We developed a method to navigate tumor neovasculature in a living tissue microenvironment, enabling relocation of a cell- or microregion-of-interest, for serial in vivo imaging. Orthotopic melanoma was grown, in immunocompetent Tie2GFP mice. Intravital multiphoton fluorescence and confocal reflectance imaging was performed, on a custom microscope with motorized stage and coordinate navigation software. A point within a Tie2GFP+ microvessel was selected for relocation. Custom software predicted target coordinates based upon reference points (tissue-embedded polystyrene beads) and baseline target coordinates. Mice were removed from the stage to make p

SUBMITTER: Dunphy MP 

PROVIDER: S-EPMC2739383 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature