Integration of evolutionary features for the identification of functionally important residues in major facilitator superfamily transporters.

ABSTRACT: The identification of functionally important residues is an important challenge for understanding the molecular mechanisms of proteins. Membrane protein transporters operate two-state allosteric conformational changes using functionally important cooperative residues that mediate long-range communication from the substrate binding site to the translocation pathway. In this study, we identified functionally important cooperative residues of membrane protein transporters by integrating sequence conservation and co-evolutionary information. A newly derived evolutionary feature, the co-evolutionary coupling number, was introduced to measure the connectivity of co-evolving residue pairs and was integrated with the sequence conservation score. We tested this method on three Major Facilitator Sup

SUBMITTER: Jeon J 

PROVIDER: S-EPMC2739438 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature