Evaluation of peak overlap in migration-time distributions determined by organelle capillary electrophoresis: Type-II error analogy based on statistical-overlap theory.

ABSTRACT: Organelles commonly are separated by capillary electrophoresis (CE) with laser-induced-fluorescence detection. Usually, it is assumed that peaks observed in the CE originate from single organelles, with negligible occurrence of peak overlap. Under this assumption, migration-time and mobility distributions are obtained by partitioning the CE into different regions and counting the number of observed peaks in each region. In this paper, criteria based on statistical-overlap theory (SOT) are developed to test the assumption of negligible peak overlap and to predict conditions for its validity. For regions of the CE having constant peak density, the numbers of peaks (i.e., intensity profiles of single organelles) and observed peaks (i.e., maxima) are modeled by probability distributions. For m

SUBMITTER: Davis JM 

PROVIDER: S-EPMC2739691 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature