Identification of dynein light chain road block-1 as a novel interaction partner with the human reduced folate carrier.

ABSTRACT: The reduced folate carrier (RFC) is a major folate transport system in mammalian cells. RFC is highly expressed in the intestine and believed to play a role in folate absorption. Studies from our laboratory and others have characterized different aspects of the intestinal folate absorption process, but little is known about possible existence of accessory protein(s) that interacts with RFC and influences its physiology and/or cell biology. We investigated this issue by employing a bacterial two-hybrid system to screen a BacterioMatch II human intestinal cDNA library using the large intracellular loop between transmembrane domains 6 and 7 of the human RFC (hRFC) as bait. Our screening has resulted in the identification of dynein light chain road block-1 (DYNLRB1) as an interacting partner w

SUBMITTER: Ashokkumar B 

PROVIDER: S-EPMC2739825 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature