Low intensity ultrasound-induced apoptosis in human gastric carcinoma cells.

ABSTRACT:

Aim

To investigate the low intensity ultrasound (US)-induced apoptosis in human gastric carcinoma cells and its potential mechanism and to suggest a new therapeutic approach to gastric carcinoma.

Methods

Human SGC-7901 gastric carcinoma cells were cultured in vitro and irradiated by low intensity US for 10 min at different intensities with different incubation times after irradiation. Morphologic changes were examined under microscope with trypan blue staining and then the percentage of early apoptotic cells was detected by flow cytometry (FCM) with double staining of fluorescein isothiocyanate (FITC)-Annexin V/propidium iodide (PI). Two-dimensional electrophoresis (2DE) was used to get the protein profile and some proteins differently expressed after US irradiation were ide

SUBMITTER: Feng Y 

PROVIDER: S-EPMC2739939 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature