Rapid identification of bacterial pathogens using a PCR- and microarray-based assay.

ABSTRACT:

Background

During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked b

SUBMITTER: Jarvinen AK 

PROVIDER: S-EPMC2741468 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature