Identification of novel endogenous antisense transcripts by DNA microarray analysis targeting complementary strand of annotated genes.

ABSTRACT:

Background

Recent transcriptomic analyses in mammals have uncovered the widespread occurrence of endogenous antisense transcripts, termed natural antisense transcripts (NATs). NATs are transcribed from the opposite strand of the gene locus and are thought to control sense gene expression, but the mechanism of such regulation is as yet unknown. Although several thousand potential sense-antisense pairs have been identified in mammals, examples of functionally characterized NATs remain limited. To identify NAT candidates suitable for further functional analyses, we performed DNA microarray-based NAT screening using mouse adult normal tissues and mammary tumors to target not only the sense orientation but also the complementary strand of the annotated genes.

Results

First, we de

SUBMITTER: Numata K 

PROVIDER: S-EPMC2741491 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature