Transgene-specific host responses in cutaneous gene therapy: the role of cells expressing the transgene.

ABSTRACT: A major issue in long-term gene therapy is host immune responses to therapeutic cells when transgene encodes a potential antigen. The nature of these responses depends on several factors including the type of cell and tissue expressing the transgene. Keratinocytes and fibroblasts, which are known to display distinct immunogenic profiles, are both potential targets for transgene expression in cutaneous gene therapy. However, whether there is an immunological advantage in targeting one cell type over the other is not known. To study the effect of cell type on transgene-specific host responses independent of antigen levels or methods of gene transfer and transplantation, we used a skin transplantation model in which transgene expression can be targeted transgene to either keratinocytes or fib

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC2741533 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature