Insulin-stimulated translocation of glucose transporter (GLUT) 12 parallels that of GLUT4 in normal muscle.

ABSTRACT:

Context

GLUT4 is the predominant glucose transporter isoform expressed in fat and muscle. In GLUT4 null mice, insulin-stimulated glucose uptake into muscle was diminished but not eliminated, suggesting that another insulin-sensitive system was present.

Objective

This study was intended to determine whether insulin caused GLUT12 translocation in muscle.

Design

Six normal volunteers had muscle biopsies before and after euglycemic insulin infusions.

Setting

Infusions and biopsies were performed in an outpatient clinic.

Participants

Subjects were nonobese, young adults with no family history of diabetes.

Main outcome measures

GLUT12, GLUT4, and GLUT1 proteins were quantified in muscle biopsy fractions. Cultured myoblasts were used to determine whether

SUBMITTER: Stuart CA 

PROVIDER: S-EPMC2741719 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature