Estrogen carcinogenesis: specific identification of estrogen-modified nucleobase in breast tissue from women.

ABSTRACT: Prolonged exposure to estrogens correlates with an increased risk for breast cancer. One explanation is that estrogen metabolites cause mutations by reacting with DNA, leading to depurination. We describe an extraction procedure and a liquid chromatographic tandem mass spectrometric (LC/MS/MS) assay to detect estrone-metabolite-modified adenine (Ade) in 100-200 mg samples of human breast tissue. To ensure reliable analyses, we used a synthetic estrone-metabolite-modified, U-(15)N-labeled Ade as an internal standard (IS). Appropriate high-pressure liquid chromatography gives sharp (approximately 5 s at half-height) and identical retention times for the analyte and the IS. In breast tissue from women with and without cancer, we found a coeluting material with similar MS/MS fragmentation as t

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC2742319 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature