Real-time label-free detection of complement activation products in human serum by white light reflectance spectroscopy.

ABSTRACT: We present a label-free, real-time sensor based on white light reflectance spectroscopy for quantitating the complement activation product C3b and its metabolites as a biomarker in human serum. Our novel sensor allows real-time monitoring of biomolecular reactions (in this case, antigen-antibody reactions) taking place on a reflective surface within a flow cell. Detection was based on monitoring the increase in film thickness caused by its immunoreaction with a specific antibody; this reaction was seen as a shift in the wavelength at which constructive interference was observed. Quantitation of C3b was achieved by immobilizing a specific mouse monoclonal antibody onto the refractive surface and monitoring the rate of the signal changes occurring during the first 60s of the immunoreaction b

SUBMITTER: Petrou PS 

PROVIDER: S-EPMC2742705 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature