Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation.

ABSTRACT: Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcr

SUBMITTER: Carlson PE 

PROVIDER: S-EPMC2742718 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature