Prenatal tobacco smoke exposure affects global and gene-specific DNA methylation.

ABSTRACT:

Rationale

Prenatal exposure to tobacco smoke increases the risk for diseases later in the child's life that may be mediated through alterations in DNA methylation.

Objectives

To demonstrate that differences in DNA methylation patterns occur in children exposed to tobacco smoke and that variation in detoxification genes may alter these associations.

Methods

Methylation of DNA repetitive elements, LINE1 and AluYb8, was measured using bisulfite conversion and pyrosequencing in buccal cells of 348 children participating in the Children's Health Study. Gene-specific CpG methylation differences associated with smoke exposure were screened in 272 participants in the Children's Health Study children using an Illumina GoldenGate panel. CpG loci that demonstrated a statisticall

SUBMITTER: Breton CV 

PROVIDER: S-EPMC2742762 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature