Time-resolved transcriptome analysis of Bacillus subtilis responding to valine, glutamate, and glutamine.

ABSTRACT: Microorganisms can restructure their transcriptional output to adapt to environmental conditions by sensing endogenous metabolite pools. In this paper, an Agilent customized microarray representing 4,106 genes was used to study temporal transcript profiles of Bacillus subtilis in response to valine, glutamate and glutamine pulses over 24 h. A total of 673, 835, and 1135 amino-acid-regulated genes were identified having significantly changed expression at one or more time points in response to valine, glutamate, and glutamine, respectively, including genes involved in cell wall, cellular import, metabolism of amino-acids and nucleotides, transcriptional regulation, flagellar motility, chemotaxis, phage proteins, sporulation, and many genes of unknown function. Different amino acid treatment

SUBMITTER: Ye BC 

PROVIDER: S-EPMC2743287 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature