MicroRNA expression pattern of undifferentiated and differentiated human embryonic stem cells.

ABSTRACT: Many of the currently established human embryonic stem (hES) cell lines have been characterized extensively in terms of their gene expression profiles and genetic stability in culture. Recent studies have indicated that microRNAs (miRNAs), a class of noncoding small RNAs that participate in the regulation of gene expression, may play a key role in stem cell self-renewal and differentiation. Using both microarrays and quantitative PCR, we report here the differences in miRNA expression between undifferentiated hES cells and their corresponding differentiated cells that underwent differentiation in vitro over a period of 2 weeks. Our results confirm the identity of a signature miRNA profile in pluripotent cells, comprising a small subset of differentially expressed miRNAs in hES cells. Exami

SUBMITTER: Lakshmipathy U 

PROVIDER: S-EPMC2743486 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature