Transcriptional effects of CRP* expression in Escherichia coli.

ABSTRACT:

Background

Escherichia coli exhibits diauxic growth in sugar mixtures due to CRP-mediated catabolite repression and inducer exclusion related to phosphotransferase system enzyme activity. Replacement of the native crp gene with a catabolite repression mutant (referred to as crp*) enables co-utilization of glucose and other sugars in E. coli. While previous studies have examined the effects of expressing CRP* mutants on the expression of specific catabolic genes, little is known about the global transcriptional effects of CRP* expression. In this study, we compare the transcriptome of E. coli W3110 (expressing wild-type CRP) to that of mutant strain PC05 (expressing CRP*) in the presence and absence of glucose.

Results

The glucose effect is significantly suppressed in strain

SUBMITTER: Khankal R 

PROVIDER: S-EPMC2743635 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature