A systems biology approach to investigate the response of Synechocystis sp. PCC6803 to a high salt environment.

ABSTRACT:

Background

Salt overloading during agricultural processes is causing a decrease in crop productivity due to saline sensitivity. Salt tolerant cyanobacteria share many cellular characteristics with higher plants and therefore make ideal model systems for studying salinity stress. Here, the response of fully adapted Synechocystis sp. PCC6803 cells to the addition of 6% w/v NaCl was investigated using proteomics combined with targeted analysis of transcripts.

Results

Isobaric mass tagging of peptides led to accurate relative quantitation and identification of 378 proteins, and approximately 40% of these were differentially expressed after incubation in BG-11 media supplemented with 6% salt for 9 days. Protein abundance changes were related to essential cellular functional alter

SUBMITTER: Pandhal J 

PROVIDER: S-EPMC2743698 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature