Verification of genes differentially expressed in neuroblastoma tumours: a study of potential tumour suppressor genes.

ABSTRACT:

Background

One of the most striking features of the childhood malignancy neuroblastoma (NB) is its clinical heterogeneity. Although there is a great need for better clinical and biological markers to distinguish between tumours with different severity and to improve treatment, no clear-cut prognostic factors have been found. Also, no major NB tumour suppressor genes have been identified.

Methods

In this study we performed expression analysis by quantitative real-time PCR (QPCR) on primary NB tumours divided into two groups, of favourable and unfavourable outcome respectively. Candidate genes were selected on basis of lower expression in unfavourable tumour types compared to favourables in our microarray expression analysis. Selected genes were studied in two steps: (1) using

SUBMITTER: Thorell K 

PROVIDER: S-EPMC2743704 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature