Hypoxic transcription gene profiles under the modulation of nitric oxide in nuclear run on-microarray and proteomics.

ABSTRACT:

Background

Microarray analysis still is a powerful tool to identify new components of the transcriptosome. It helps to increase the knowledge of targets triggered by stress conditions such as hypoxia and nitric oxide. However, analysis of transcriptional regulatory events remain elusive due to the contribution of altered mRNA stability to gene expression patterns as well as changes in the half-life of mRNAs, which influence mRNA expression levels and their turn over rates. To circumvent these problems, we have focused on the analysis of newly transcribed (nascent) mRNAs by nuclear run on (NRO), followed by microarray analysis.

Results

We identified 196 genes that were significantly regulated by hypoxia, 85 genes affected by nitric oxide and 292 genes induced by the cotreatme

SUBMITTER: Igwe EI 

PROVIDER: S-EPMC2743718 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature