Microarray cluster analysis of irradiated growth plate zones following laser microdissection.

ABSTRACT:

Purpose

Genes and pathways involved in early growth plate chondrocyte recovery after fractionated irradiation were sought as potential targets for selective radiorecovery modulation.

Materials and methods

Three groups of six 5-week male Sprague-Dawley rats underwent fractionated irradiation to the right tibiae over 5 days, totaling 17.5 Gy, and then were killed at 7, 11, and 16 days after the first radiotherapy fraction. The growth plates were collected from the proximal tibiae bilaterally and subsequently underwent laser microdissection to separate reserve, perichondral, proliferative, and hypertrophic zones. Differential gene expression was analyzed between irradiated right and nonirradiated left tibia using RAE230 2.0 GeneChip microarray, compared between zones and time p

SUBMITTER: Damron TA 

PROVIDER: S-EPMC2743722 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature