Maternal influence of prolyl endopeptidase on fat mass of adult progeny.

ABSTRACT:

Background

Maternal genotype has lifetime effects on progeny, but few specific genes, and no proteases, are known to underlie maternal effects. Prolyl endopeptidase (PREP) is a serine protease with putative substrates that regulate appetite or milk production.

Objective

To test effects of PREP on obesity phenotypes in mice.

Design

Mice with a gene trap (GT) of PREP (PREP(gt/gt)) on the C57BL/6J (B6) background were generated. Minimal PREP protein was detected by western blot. In Experiment 1, direct effects of PREP were measured in littermate mice derived from intercrosses of heterozygotes (PREP(WT/gt)). In Experiment 2, maternal effects of PREP were measured in reciprocal crosses of heterozygous (PREP(WT/gt)) and wild-type (WT) (PREP(WT/WT)) males and females. DIETS:

SUBMITTER: Warden CH 

PROVIDER: S-EPMC2743752 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature