Functional evidence for the expression of P2X1, P2X4 and P2X7 receptors in human lung mast cells.

ABSTRACT:

Background and purpose

P2X receptors are widely expressed in cells of the immune system with varying functions. This study sought to characterize P2X receptor expression in the LAD2 human mast cell line and human lung mast cells (HLMCs).

Experimental approach

Reverse transcriptase polymerase chain reaction (RT-PCR) and patch clamp studies were used to characterize P2X expression in mast cells using a range of pharmacological tools.

Key results

RT-PCR revealed P2X1, P2X4 and P2X7 transcripts in both cell types; mRNA for P2X6 was also detected in LAD2 cells. Under whole-cell patch clamp conditions, rapid application of ATP (1-1000 microM) to cells clamped at -60 mV consistently evoked inward currents in both types of cells. Brief application of ATP (1 s) evoked a rapidl

SUBMITTER: Wareham K 

PROVIDER: S-EPMC2743840 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature