Efflux pump gene hefA of Helicobacter pylori plays an important role in multidrug resistance.

ABSTRACT:

Aim

To determine whether efflux systems contribute to multidrug resistance of H pylori.

Methods

A chloramphenicol-induced multidrug resistance model of six susceptible H pylori strains (5 isolates and H pylori NCTC11637) was developed. Multidrug-resistant (MDR) strains were selected and the minimal inhibitory concentration (MIC) of erythromycin, metronidazole, penicillin G, tetracycline, and ciprofloxacin in multidrug resistant strains and their parent strains was determined by agar dilution tests. The level of mRNA expression of hefA was assessed by fluorescence real-time quantitative PCR. A H pylori LZ1026 knockout mutant (delta H pylori LZ1026) for (putative) efflux protein was constructed by inserting the kanamycin resistance cassette from pEGFP-N2 into hefA, and its sus

SUBMITTER: Liu ZQ 

PROVIDER: S-EPMC2744013 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature