Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.

ABSTRACT:

Background

The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming.

Methodology/principal findings

Here, we report identification and isolation of a subpopulation of human dermal fibroblasts that express the pluripotency marker stage specific embryonic antigen 3 (SSEA3). Fibroblasts that expressed SSEA3 demonstrated an enhanced iPSC generation efficiency, while no iPSC derivation was obtained from the fibroblasts that did not express SSEA3. Transcriptional analysis revealed NANOG expression was significantly increased in the SSEA3 expressing fibroblasts, suggest

SUBMITTER: Byrne JA 

PROVIDER: S-EPMC2744017 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature