Poldip2, a novel regulator of Nox4 and cytoskeletal integrity in vascular smooth muscle cells.

ABSTRACT:

Rationale

NADPH oxidases (Noxes) regulate vascular physiology and contribute to the pathogenesis of vascular disease. In vascular smooth muscle cells (VSMCs), the interactions of individual Nox homologs with regulatory proteins are poorly defined.

Objective

The objective of this study was to identify novel NADPH oxidase regulatory proteins.

Methods and results

Using a yeast 2-hybrid screen, we identified a novel p22phox binding partner, Poldip2, and demonstrated that it associates with p22phox, NADPH oxidase (Nox)1, and Nox4 and colocalizes with p22phox at sites of Nox4 localization. Poldip2 increases Nox4 enzymatic activity by 3-fold and positively regulates basal reactive oxygen species production in VSMCs (O2(.-): 86.3+/-15.6% increase; H2O2: 40.7+/-4.5% increase).

SUBMITTER: Lyle AN 

PROVIDER: S-EPMC2744198 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature