Combination of on-chip field amplification and bovine serum albumin sweeping for ultrasensitive detection of green fluorescent protein.

ABSTRACT: We report a highly effective on-chip preconcentration method by combining field-amplified sample injection (FASI) and bovine serum albumin (BSA) sweeping for ultrasensitive detection of green fluorescent protein (GFP) on a simple cross-channel microchip device. With the formation of a stagnant sample/running buffer boundary by balancing the hydrodynamic flow and the electro-osmotic flow (EOF), GFP molecules can be continuously injected into the sample loading channel and stacked. We have also demonstrated that BSA is a very effective pseudo-stationary phase for sweeping concentration of proteins in comparison to the commonly used micelles. The combination of FASI and BSA sweeping yields a concentration factor of 3570 and a limit of detection of 8.4 pM for GFP. Using this method, we have se

SUBMITTER: Pan Q 

PROVIDER: S-EPMC2744439 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature