Temporal Notch activation through Notch1a and Notch3 is required for maintaining zebrafish rhombomere boundaries.

ABSTRACT: In vertebrates, hindbrain is subdivided into seven segments termed rhombomeres and the interface between each rhombomere forms the boundary. Similar to the D/V boundary formation in Drosophila, Notch activation has been shown to regulate the segregation of rhombomere boundary cells. Here we further explored the function of Notch signaling in the formation of rhombomere boundaries. By using bodipy ceramide cell-labeling technique, we found that the hindbrain boundary is formed initially in mib mutants but lost after 24 hours post-fertilization (hpf). This phenotype was more severe in mib(ta52b) allele than in mib(tfi91) allele. Similarly, injection of su(h)-MO led to boundary defects in a dosage-dependent manner. Boundary cells were recovered in mib(ta52b) mutants in the hdac1-deficient bac

SUBMITTER: Qiu X 

PROVIDER: S-EPMC2744777 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature