Integration of small-angle X-ray scattering data into structural modeling of proteins and their assemblies.

ABSTRACT: A major challenge in structural biology is to determine the configuration of domains and proteins in multidomain proteins and assemblies, respectively. All available data should be considered to maximize the accuracy and precision of these models. Small-angle X-ray scattering (SAXS) efficiently provides low-resolution experimental data about the shapes of proteins and their assemblies. Thus, we integrated SAXS profiles into our software for modeling proteins and their assemblies by satisfaction of spatial restraints. Specifically, we modeled the quaternary structures of multidomain proteins with structurally defined rigid domains as well as quaternary structures of binary complexes of structurally defined rigid proteins. In addition to SAXS profiles and the component structures, we used st

SUBMITTER: Forster F 

PROVIDER: S-EPMC2745287 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature