Presence of activating KRAS mutations correlates significantly with expression of tumour suppressor genes DCN and TPM1 in colorectal cancer.

ABSTRACT:

Background

Despite identification of the major genes and pathways involved in the development of colorectal cancer (CRC), it has become obvious that several steps in these pathways might be bypassed by other as yet unknown genetic events that lead towards CRC. Therefore we wanted to improve our understanding of the genetic mechanisms of CRC development.

Methods

We used microarrays to identify novel genes involved in the development of CRC. Real time PCR was used for mRNA expression as well as to search for chromosomal abnormalities within candidate genes. The correlation between the expression obtained by real time PCR and the presence of the KRAS mutation was investigated.

Results

We detected significant previously undescribed underexpression in CRC for genes SLC26A3

SUBMITTER: Mlakar V 

PROVIDER: S-EPMC2745427 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature