Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.

ABSTRACT:

Background

The prostate stroma is a key mediator of epithelial differentiation and development, and potentially plays a role in the initiation and progression of prostate cancer. The tumor-associated stroma is marked by increased expression of CD90/THY1. Isolation and characterization of these stromal cells could provide valuable insight into the biology of the tumor microenvironment.

Methods

Prostate CD90+ stromal fibromuscular cells from tumor specimens were isolated by cell-sorting and analyzed by DNA microarray. Dataset analysis was used to compare gene expression between histologically normal and tumor-associated stromal cells. For comparison, stromal cells were also isolated and analyzed from the urinary bladder.

Results

The tumor-associated stromal cells were f

SUBMITTER: Pascal LE 

PROVIDER: S-EPMC2745432 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature