Identification of an unusual pattern of global gene expression in group B Streptococcus grown in human blood.

ABSTRACT: Because passage of the bacterium to blood is a crucial step in the pathogenesis of many group B Streptococcus (GBS) invasive infections, we recently conducted a whole-genome transcriptome analysis during GBS incubation ex vivo with human blood. In the current work, we sought to analyze in detail the difference in GBS gene expression that occurred in one blood sample (donor A) relative to other blood samples. We incubated GBS strain NEM316 with fresh heparinized human blood obtained from healthy volunteers, and analyzed GBS genome expression and cytokine production. Principal component analysis identified extensive clustering of the transcriptome data among all samples at time 0. In striking contrast, the whole bacterial gene expression in the donor A blood sample was significantly differen

SUBMITTER: Mereghetti L 

PROVIDER: S-EPMC2745576 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature