Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.

ABSTRACT:

Background

MicroRNAs are required for maintenance of pluripotency as well as differentiation, but since more microRNAs have been computationally predicted in genome than have been found, there are likely to be undiscovered microRNAs expressed early in stem cell differentiation.

Methodology/principal findings

SOLiD ultra-deep sequencing identified >10(7) unique small RNAs from human embryonic stem cells (hESC) and neural-restricted precursors that were fit to a model of microRNA biogenesis to computationally predict 818 new microRNA genes. These predicted genomic loci are associated with chromatin patterns of modified histones that are predictive of regulated gene expression. 146 of the predicted microRNAs were enriched in Ago2-containing complexes along with 609 known microR

SUBMITTER: Goff LA 

PROVIDER: S-EPMC2745660 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature