Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application.

ABSTRACT:

Background

Poly-beta-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported for the first time that a stress-induced system enabled E. coli, a non-PHB producer, to mobilize PHB in vivo by mimicking natural PHB accumulation bacteria.

Results

The successful expression of PHB biosynthesis and PHB depolymerase genes in E. coli was confirmed by PHB production and 3-hydroxybutyrate secretion. Starvation experiment demonstrated that the complete PHB mobilization system in E. coli served as an intracellular energy and carbon storage system, which increased the survival rate of the host when carbon resources were limited. Stress tolerance experiment indicated that E. coli strai

SUBMITTER: Wang Q 

PROVIDER: S-EPMC2746179 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature