Sphingosine-1-phosphate receptor-2 regulates expression of smooth muscle alpha-actin after arterial injury.

ABSTRACT:

Objective

This study tests the hypothesis that S1P2R regulates expression of SMC differentiation genes after arterial injury.

Methods and results

Carotid ligation injury was performed in wild-type and S1P2R-null mice. At various time points after injury, expression of multiple SMC differentiation genes, myocardin, and S1P receptors (S1P1R, S1P2R, and S1P3R) was measured by quantitative PCR. These experiments demonstrate that at day 7 after injury, S1P2R specifically regulates expression of smooth muscle alpha-actin (SMA) and that this is not mediated by changes in expression of myocardin or any of the S1PRs. In vitro studies using carotid SMCs prepared from wild-type and S1P2R-null mice show that S1P stimulates expression of all SMC-differentiation genes tested, but S1P2R si

SUBMITTER: Grabski AD 

PROVIDER: S-EPMC2746263 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature