In vivo imaging of autologous islet grafts in the liver and under the kidney capsule in non-human primates.

ABSTRACT:

Objective

As islet transplantation begins to show promise as a clinical method, there is a critical need for reliable, noninvasive techniques to monitor islet graft survival. Previous work in our laboratory has shown that human islets labeled with a superparamagnetic iron oxide contrast agent and transplanted into mice could be detected by magnetic resonance imaging (MRI). The potential translation of these findings to the clinical situation requires validation of our methodology in a non-human primate model, which we have now carried out in baboons (Papio hamadryas) and reported here.

Research design and methods

For islet labeling, we adapted the Food and Drug Administration-approved superparamagnetic iron oxide contrast agent, Feridex, which is used clinically for liver im

SUBMITTER: Medarova Z 

PROVIDER: S-EPMC2746764 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature