Intracellular biology and virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages.

ABSTRACT: Summary The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen, whose virulence requires proliferation inside host cells, including macrophages. Here we have performed a global transcriptional profiling of the highly virulent F. tularensis ssp. tularensis Schu S4 strain during its intracellular cycle within primary murine macrophages, to characterize its intracellular biology and identify pathogenic determinants based on their intracellular expression profiles. Phagocytosed bacteria rapidly responded to their intracellular environment and subsequently altered their transcriptional profile. Differential gene expression profiles were revealed that correlated with specific intracellular locale of the bacteria. Upregulation of general and oxidative stres

SUBMITTER: Wehrly TD 

PROVIDER: S-EPMC2746821 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature