Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP.

ABSTRACT: Most human protein-encoding genes contain multiple exons that are spliced together, frequently in alternative arrangements, by the spliceosome. It is established that U1 snRNP is an essential component of the spliceosome, in human consisting of RNA and ten proteins, several of which are post-translationally modified and exist as multiple isoforms. Unresolved and challenging to investigate are the effects of these post translational modifications on the dynamics, interactions and stability of the particle. Using mass spectrometry we investigate the composition and dynamics of the native human U1 snRNP and compare native and recombinant complexes to isolate the effects of various subunits and isoforms on the overall stability. Our data reveal differential incorporation of four protein isofor

SUBMITTER: Hernandez H 

PROVIDER: S-EPMC2747018 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature