Bone marrow progenitor cells repair rat hepatic sinusoidal endothelial cells after liver injury.

ABSTRACT:

Background & aims

Damage to hepatic sinusoidal endothelial cells (SECs) initiates sinusoidal obstruction syndrome (SOS), which is most commonly a consequence of myeloablative chemoirradiation or ingestion of pyrrolizidine alkaloids such as monocrotaline (Mct). This study examines whether SECs are of bone marrow origin, whether bone marrow repair can be a determinant of severity of liver injury, and whether treatment with progenitor cells is beneficial.

Methods

Mct-treated female rats received infusion of male whole bone marrow or CD133(+) cells at the peak of sinusoidal injury. The Y chromosome was identified in isolated SECs by fluorescent in situ hybridization. Bone marrow suppression was induced by irradiation of both lower extremities with shielding of the abdomen.

Re

SUBMITTER: Harb R 

PROVIDER: S-EPMC2747805 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature